GENERAL PATHOLOGY, HISTOPATHOLOGIC AND CYTOLOGIC TECHNIQUES SYLLABUS

UNIVERSIDAD DE ZAMBOANGA
SCHOOL OF ALLIED MEDICINE
Medical Technology Department

COURSE SYLLABUS

GENERAL PATHOLOGY, HISTOPATHOLOGIC AND CYTOLOGIC TECHNIQUES
S.Y. 2009-2010 (First Semester)

Course Description : This is divided into three. The first part deals with
the study of basic disease processes, correlating the etiology of disease with the course of development of anatomic and clinical changes brought about by the disease. The second part is a study of the histologic techniques essential in the production of histologic slides for the diagnosis of diseases including special staining procedures. The third part is the study and identification of cells in the diagnosis of diseases using cytologic technique.

Course Credit : 3 units (2 units lecture and 1 unit laboratory)
Contact Hours : 1 hour lecture and 3 hours laboratory per week
Total of 36 hours lecture and 54 hours laboratory per
semester
Prerequisite : Histology
Placement : Fourth Year, First Semester
Course Objectives : At the end of the course, the students should be able to;
1. discuss the basic concept of disease formation;
2. explain the physiologic changes brought about by
the various diseases;
3. discuss step by step procedure in tissue
preparation;
4. recognize the concepts of quality assurance
program in Histopathology laboratory;
5. Acquire the necessary skills required in the
preparation of slides for microscopic
examination of tissues from fresh state to
mounted state.
6. apply the knowledge acquired about diseases in
daily living;
7. manifest the following values: honesty, critical
thinking, empathy and value for life.

Teaching Methodology: Brain Storming, Demonstration, Group Discussion, Lecture

Course Outline
PRE-MID
Contact Hours: 9 hours lecture: 14 hours laboratory
I. Review : Fundamentals of Normal Histology
• Normal cell structure
• Cell in tissues, types and sub-types of tissues and their location
• Cellular adaptation: terminologies (atrophy, hypertrophy, hyperplasia, dysplasia, hypoplasia, agenesis etc.

II. Introduction to Pathology
• Definition of terms (pathology, pathogenesis, pathologist, medical technologist, autopsy, biopsy etc.)
• Divisions of p\Pathology
Gross and Microscopic Pathology
Anatomic Pathology
Clinical Pathology
• Nature of Diseases
Definition of terms (disease, health etiology etc.)
Describe manifestation of disease
Signs
Symptoms
Course of Disease
• Factors predisposing an individual to disease
Definition of terms (disease, health etiology etc.)
III. Etiology of Disease
• Classification of diseases according to the basic etiologic mechanisms, their features and laboratory findings
Hereditary disorders and congenital anomalies
Circulatory disorders
Metabolic disorders and disturbance
Deficiency diseases
Disorders of the immune system
Neoplasms
Disorders caused by air pollution, chemical and physical injuries
Extrinsic
Intrinsic
MID TERM
Contact Hours: 9 hours lecture: 13 hours laboratory
Routine Histotechniques
• Collection and Handling of Histological Specimens
• Steps in tissue processing
 Fixation
-definition
-good characteristics of fixative agents
-components of fixative agents
-steps in fixation
-uses of fixative agents
 Decalcification
-definition
-good characteristics of decalcifying agents
-components of decalcifying agents
-steps in decalcification
-uses of decalcifying agents
 Dehydration
-definition
-good characteristics of dehydrating agents
-components of dehydrating agents
-steps in dehydration
-uses of dehydrating agents

PRE FINAL TERM
Contact Hours: 9 hours lecture: 14 hours laboratory
Routine Histotechniques
Steps in tissue processing
 Clearing
-definition
-good characteristics of clearing agents
-components of clearing agents
-steps in clearing tissues
-uses of clearing agents
 Impregnation
-definition
-good characteristics
-components
-steps in impregnation
-uses
 Embedding
-definition
-good characteristics
-components
-steps
-uses
 Trimming and Cutting of Tissue Sections
-definition
-good characteristics
-steps

FINAL TERM
Contact Hours: 9 hours lecture: 13 hours laboratory
Routine Histotechniques
Steps in tissue processing
 Mounting of tissue sections

 Staining
-definition
-good characteristics
-components
-steps
-uses
 Difficulties encountered during tissue processing and the corresponding remedies
 Special processing techniques
-Rapid processing techniques
-Preparation of bone marrow section
-Enzyme histo
 Exfoliative cytology
-Definition of terms
-Collection of specimens
-Preparation, fixation, staining of smears
-vaginal cytology
LABORATORY EXERCISES;
1. Laboratory safety
2. Instrumentation
3. Paper Mold Making
4. Sharpening of Knife
5. General Procedure for Tissue Processing
6. Trimming
7. Rotary Microtome
8. Adhesive Preparation
9. Section Cutting
10. Staining of Sections
11. Mounting
12. Labeling
13. Pap’s Staining

REFERENCES: Gregorios, J. (2006). Histopathologic Techniques, 2nd Ed.Goodwill
Trading Co.,Inc. Philippines
Raphael, S. Lynch’s Medical Laboratory Technology, 4th Ed. MW
Publishing Inc. USA